病毒載體的啟動子元件決定了病毒表達的特異性,為了靶向性研究或基因治療,我們需要不斷開發適合特異性細胞或組織的啟動子,並不斷優化。目前,尊龙凯时基礎研發部着重針對不同組織或器官中的特定細胞進行啟動子優化、篩選,同時也歡迎啟動子研發合作項目。
AAV8-TGB-Fabp1[1](TG乙肝臟特異性啟動子)
AAV-CaMKIIα-NpHR-EYFP[2](CaMKIIα投射神經元特異性啟動子)
AAV-Syn-CAPON-L-GFP[3](Syn成熟神經元特異性啟動子)
AAV-GFAP- AAV–Ascl1/mCherry [4](GFAP星形膠質細胞特異性啟動子)
參考文獻:
[1] Pi H. et al., (2019) Long-term exercise prevents hepatic steatosis: a novel role of FABP1 in regulation of autophagy-lysosomal machinery. FASEB J. doi: 10.1096/fj.201900812R.
[2] Li YD, et al., (2017) A distinct entorhinal cortex to hippo-campal CA1 direct circuit for olfactory associative learning. Nature Neuroscience.
[3] Zhu L J, et al., (2014) CAPON-nNOS coupling can serve as a target for developing new anxiolytics. Nature Medicine.
[4] Liu Y G, et al., (2015) Ascl1 Converts Dorsal Midbrain Astrocytes into Functional Neurons In Vivo. J. Neuroscience.